| Microorganism name |
Kluyveromyces marxianus
|
| Target proteins |
Soy leghemoglobin (Tian et al., 2024)
|
| Wild-type or GMO |
GMO (Tian et al., 2024)
|
| Production mode (intracellular/extracellular) |
Intracellular (Tian et al., 2024)
|
| Protein yield (g/L or g/g?) |
7.27 g/L (Tian et al., 2024)
|
| Temperature used in study |
32°C (Tian et al., 2024)
|
| pH used in study |
pH 5.5 (Tian et al., 2024)
|
| C & N source |
Glucose, glycine (Tian et al., 2024)
|
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
No FDA GRAS approval in US, not allowed in Canada |
| Companies |
NA |
| Publications/references |
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Tian, T., Wu, X., Wu, P., Lu, X., Wang, Q., Lin, Y., Liu, C., Zhou, J., Yu, Y., & Lu, H. (2024). High-level expression of leghemoglobin in Kluyveromyces marxianus by remodeling the heme metabolism pathway. Frontiers in Bioengineering and Biotechnology, 11. https://doi.org/10.3389/fbioe.2023.1329016
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Dong, L., Wu, Y., Li, M., Zhang, C., Cao, J., Ledesma-Amaro, R., Zhao, W., & Kang, D. (2025). Exploring the Fermentation Potential of Kluyveromyces marxianus NS127 for Single-Cell Protein Production. Fermentation, 11(2), 70. https://doi.org/10.3390/fermentation11020070
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Bilal, M., Ji, L., Xu, Y., Xu, S., Lin, Y., Iqbal, H. M. N., & Cheng, H. (2022). Bioprospecting Kluyveromyces marxianus as a Robust Host for Industrial Biotechnology. Frontiers in Bioengineering and Biotechnology, 10. https://doi.org/10.3389/fbioe.2022.851768
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Yadav, J. S. S., Bezawada, J., Elharche, S., Yan, S., Tyagi, R. D., & Surampalli, R. Y. (2013). Simultaneous single-cell protein production and COD removal with characterization of residual protein and intermediate metabolites during whey fermentation by K. marxianus. Bioprocess and Biosystems Engineering, 37(6), 1017–1029. https://doi.org/10.1007/s00449-013-1072-6
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Amara, A. A., & El-Baky, N. A. (2023). Fungi as a Source of Edible Proteins and Animal Feed. Journal of Fungi, 9(1), 73. https://doi.org/10.3390/jof9010073
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Zhou, J., Zhu, P., Hu, X., Lu, H., & Yu, Y. (2018). Improved secretory expression of lignocellulolytic enzymes in Kluyveromyces marxianus by promoter and signal sequence engineering. Biotechnology for Biofuels, 11(1). https://doi.org/10.1186/s13068-018-1232-7
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Baptista, M., & Domingues, L. (2022). Kluyveromyces marxianus as a microbial cell factory for lignocellulosic biomass valorisation. Biotechnology Advances, 60, 108027. https://doi.org/10.1016/j.biotechadv.2022.108027
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